PLOS ONE: A Simple and Accurate Two-Step Long DNA Sequences Synthesis Strategy to Improve Heterologous Gene Expression in Pichia
![Selective control of primer usage in multiplex one-step reverse transcription PCR | BMC Molecular Biology | Full Text Selective control of primer usage in multiplex one-step reverse transcription PCR | BMC Molecular Biology | Full Text](https://media.springernature.com/lw685/springer-static/image/art%3A10.1186%2F1471-2199-10-113/MediaObjects/12867_2009_Article_481_Fig1_HTML.jpg)
Selective control of primer usage in multiplex one-step reverse transcription PCR | BMC Molecular Biology | Full Text
![A robust two-step PCR method of template DNA production for high-throughput cell-free protein synthesis | SpringerLink A robust two-step PCR method of template DNA production for high-throughput cell-free protein synthesis | SpringerLink](https://media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs10969-007-9038-z/MediaObjects/10969_2007_9038_Fig3_HTML.gif)
A robust two-step PCR method of template DNA production for high-throughput cell-free protein synthesis | SpringerLink
![Cloning‐free template DNA preparation for cell‐free protein synthesis via two‐step PCR using versatile primer designs with short 3′‐UTR - Nomoto - 2018 - Genes to Cells - Wiley Online Library Cloning‐free template DNA preparation for cell‐free protein synthesis via two‐step PCR using versatile primer designs with short 3′‐UTR - Nomoto - 2018 - Genes to Cells - Wiley Online Library](https://onlinelibrary.wiley.com/cms/asset/e7c4dc2e-c005-4951-8f7b-0a3c0abd7bde/gtc12547-fig-0002-m.jpg)
Cloning‐free template DNA preparation for cell‐free protein synthesis via two‐step PCR using versatile primer designs with short 3′‐UTR - Nomoto - 2018 - Genes to Cells - Wiley Online Library
![2-step PCR method for deep sequencing preparation of libraries. PCR... | Download Scientific Diagram 2-step PCR method for deep sequencing preparation of libraries. PCR... | Download Scientific Diagram](https://www.researchgate.net/publication/273780840/figure/fig8/AS:341285459251201@1458380202355/2-step-PCR-method-for-deep-sequencing-preparation-of-libraries-PCR-reactions-are-shown.png)
2-step PCR method for deep sequencing preparation of libraries. PCR... | Download Scientific Diagram
![Agronomy | Free Full-Text | A Two-Step PCR Protocol Enabling Flexible Primer Choice and High Sequencing Yield for Illumina MiSeq Meta-Barcoding Agronomy | Free Full-Text | A Two-Step PCR Protocol Enabling Flexible Primer Choice and High Sequencing Yield for Illumina MiSeq Meta-Barcoding](https://www.mdpi.com/agronomy/agronomy-11-01274/article_deploy/html/images/agronomy-11-01274-g003.png)
Agronomy | Free Full-Text | A Two-Step PCR Protocol Enabling Flexible Primer Choice and High Sequencing Yield for Illumina MiSeq Meta-Barcoding
![High-throughput sequencing of multiple amplicons for barcoding and integrative taxonomy | Scientific Reports High-throughput sequencing of multiple amplicons for barcoding and integrative taxonomy | Scientific Reports](https://media.springernature.com/m685/springer-static/image/art%3A10.1038%2Fsrep41948/MediaObjects/41598_2017_Article_BFsrep41948_Fig1_HTML.jpg)
High-throughput sequencing of multiple amplicons for barcoding and integrative taxonomy | Scientific Reports
![Scheme of a standard two-step PCR approach. In a two-step PCR strategy... | Download Scientific Diagram Scheme of a standard two-step PCR approach. In a two-step PCR strategy... | Download Scientific Diagram](https://www.researchgate.net/profile/Sigrid-Schaper/publication/279967454/figure/fig3/AS:324903183110172@1454474363747/Scheme-of-a-standard-two-step-PCR-approach-In-a-two-step-PCR-strategy-A-a-PCR-product.png)